Dexmethylphenidate, otherwise known as d-threo-methylphenidate (D-TMP: (R,R)-(+)-Methyl 2-phenyl-2-(2-piperidyl)acetate), is the dextrorotatory enantiomer of Methylphenidate. In order to assess the safety and efficacy of Dexmethylphenidate, a sensitive analytical method is needed. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay capable of measuring analyte in human plasma was developed and validated to support clinical studies. Venlafaxine Hydrochloride was employed as an Internal Standard. The method of extraction of drug from plasma was done by solid phase extraction (SPE) using DVB-LP polymeric SPE cartridges (30mg, 1ml capacity). Chromatography was performed on X-terra RP C18 (4.6×150mm, 5µm) column using mobile phase constituting Methanol: 5mM ammonium acetate (pH4.00)- 90:10, and detected by mass spectrum (AB Sciex) MRM mode was used for MS/MS detection, Analytical software version is Analyst 1.4.2/1.5.1. The method was linear over a concentration range of 0.201ng/ml to 80.434ng/ml, with a Correlation coefficient (r) was found to be more than 0.999. The mean recovery of dexmethylphenidate is 65.83 % at an overall precision of 7.38 %, with lower limit of quantification set at 0.201ng/ml. This method was simple, fast, specific and exhibited excellent ruggedness.
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